How Does AAO Wastewater Treatment Really Work

By Nancy on Feb-04-2026

AAO wastewater treatment is a biological process designed to remove harmful substances from water. This method uses three main stages, each with a specific function in cleaning wastewater:

  • Creates different biochemical environments to remove carbon, nitrogen, and phosphorus

  • Optimizes pollutant removal and manages operational costs

  • Evolved as the most common approach for nutrient removal

Stage

Contribution to Pollutant Removal

Anaerobic

Releases phosphorus and breaks down organic matter

Anoxic

Removes nitrogen through denitrification

Aerobic

Converts ammonium into nitrate

Key Takeaways

  • AAO wastewater treatment uses three stages: anaerobic, anoxic, and oxic, to effectively remove harmful substances from water.

  • The anaerobic stage breaks down organic matter and releases phosphorus, while the anoxic stage converts nitrate into nitrogen gas, reducing pollution.

  • The oxic stage uses oxygen to finish breaking down pollutants and absorb remaining phosphorus, ensuring clean water for communities.

AAO wastewater treatment stages

Anaerobic stage overview

The anaerobic stage is the first step in AAO wastewater treatment. In this stage, oxygen is absent. Microorganisms break down organic matter and release phosphorus. This process uses internal carbon sources, such as poly-β‑hydroxy alkanoates (PHA) and glycogen (Gly), to drive denitrification. These compounds help remove nitrogen without needing extra chemicals.

The anaerobic stage of the AAO wastewater treatment process utilizes internal carbon sources for denitrification, allowing nitrogen removal without the need for external carbon sources. This process is facilitated by the storage and utilization of poly-β‑hydroxy alkanoates (PHA) and glycogen (Gly), which are the main intracellular carbon sources driving denitrification in the post-anoxic zone.

Several types of microorganisms play important roles here. Phosphorus-accumulating organisms (PAOs) remove phosphorus. Glycogen-accumulating organisms (GAOs) compete with PAOs for organic matter and can also perform denitrification. Ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) help with nitrogen transformations.

Microorganism Type

Role in Anaerobic Stage

Ammonia-oxidizing bacteria (AOB)

Oxidation of ammonia to nitrite

Nitrite-oxidizing bacteria (NOB)

Oxidation of nitrite to nitrate

Phosphorus-accumulating organisms (PAOs)

Responsible for phosphorus removal

Glycogen-accumulating organisms (GAOs)

Compete with PAOs for organic matter; can perform denitrification

The anaerobic stage targets several pollutants for removal. The table below shows the efficiency of this stage:

Pollutant Type

Removal Efficiency

BOD5

90%–95%

Total Nitrogen

Above 70%

Phosphorus

Around 90%

Anoxic stage overview

The anoxic stage follows the anaerobic stage. Here, oxygen is still absent, but nitrate and nitrite are present. Denitrifying bacteria use organic matter to convert nitrate and nitrite into nitrogen gas. This process removes nitrogen from the water and helps prevent water pollution in rivers and lakes.

The anoxic stage is different from the anaerobic stage. While the anaerobic stage focuses on breaking down organic matter and releasing phosphorus, the anoxic stage is essential for denitrification. Denitrifying bacteria convert nitrate and nitrite into nitrogen gas, which escapes into the atmosphere.

The removal rate of nitrate nitrogen in the anoxic stage can exceed 90%. This high efficiency makes the anoxic stage a key part of AAO wastewater treatment.

In the anoxic zone, denitrifying bacteria play a vital role. They convert nitrate (NO₃⁻) into nitrogen gas (N₂) through several steps. This process reduces nitrogen levels and helps prevent problems like eutrophication in lakes and rivers. Adding carbon sources in the anoxic zone can further lower nitrate levels and control the production of nitrous oxide (N2O).

Oxic stage overview

The oxic stage is the final step in AAO wastewater treatment. In this stage, oxygen is present. Microorganisms use oxygen to break down organic matter. Ammonia is converted into nitrate through a process called nitrification. Phosphorus-accumulating organisms (PAOs) absorb phosphorus from the water.

In the aerobic oxic zone, the main activities include the oxidation of organic matter, nitrification of ammonia into nitrate, and enhanced uptake of phosphorus by PAOs. This stage ensures that most remaining pollutants are removed before the water is released or reused.

Pollutant Type

Mechanism Involved

Nitrogen

Nitrification (conversion of NH4-N to NO2- and NO3-)

Phosphorus

Biological phosphorus removal (absorption by microorganisms)

Organic Pollutants

Oxidation and decomposition by microorganisms

The AAO process is recognized as the most prevalent biological configuration for nutrient removal, effectively achieving the simultaneous removal of organics, nitrogen, and phosphorus. It has been shown that employing denitrifying phosphorus removal (DPR) within the AAO process allows for this simultaneous removal with minimal reliance on external carbon sources, thus supporting the claim of minimal chemical use.

AAO wastewater treatment is widely used because it removes nitrogen and phosphorus efficiently with little need for chemicals. Companies like Mejec have advanced this field by developing intelligent, decentralized solutions for industries such as healthcare, education, hospitality, and real estate. Their DeepDragon™ smart operation technology and high-precision manufacturing help ensure that treatment systems meet strict standards for compliance, efficiency, and environmental protection. This expertise supports the reliable performance of AAO wastewater treatment in many settings.

Effectiveness and results

How the stages interact

The three stages in AAO wastewater treatment work together to remove pollutants efficiently. Each stage creates the right environment for specific microorganisms to do their job. The anaerobic stage releases phosphorus and breaks down organic matter. The anoxic stage removes nitrogen by turning nitrate into nitrogen gas. The oxic stage uses oxygen to finish breaking down pollutants and absorb phosphorus. When these stages interact, they boost the removal of nitrogen and phosphorus through connected pathways.

Parameter

Value

Sludge reflux ratio

200%

Temperature

25 °C

C/N ratio

8

COD removal ratio

92.80 ± 2.24%

NH4+-N removal ratio

97.38 ± 0.89%

TN removal ratio

88.97 ± 2.47%

PO43--P removal ratio

94.33 ± 3.27%

Aeration volume savings

23.08%

This table shows how the stages work together to achieve high removal rates for key pollutants.

Pollutants removed

AAO wastewater treatment is highly effective at removing organic matter, nitrogen, and phosphorus. Compared to other biological methods, it consistently achieves high removal rates.

Pollutant Type

AAO Effectiveness

Other Biological Methods Effectiveness

Organic Matter

High

Varies

Nitrogen

High

Varies

Phosphorus

High

Varies

Typical removal rates include:

  • Nitrogen removal efficiency: up to 92%

  • Phosphorus removal efficiency: up to 95%

  • Total inorganic nitrogen in effluent: ≤3 mg/L

  • Phosphorus in effluent: ≤0.2 mg/L

Expected outcomes

AAO wastewater treatment helps communities meet strict water quality standards. It reduces pollutants, saves energy, and lowers chemical use. Many municipalities and industries choose this process because it is reliable and cost-effective. The process also supports clean water initiatives by improving water quality and promoting conservation.

Advanced solutions, such as those developed by Mejec, use smart operation technology to optimize AAO systems. These systems help ensure compliance, efficiency, and ecological balance in different settings, from hospitals to schools and public spaces.

AAO wastewater treatment uses three biological stages to clean water.

  • The anaerobic stage removes most organic matter and phosphorus.

  • The anoxic stage converts nitrate into nitrogen gas.

  • The oxic stage finishes breaking down pollutants and supports estrogen degradation.
    This process ensures reliable, clean water for communities and industries.

FAQ

What does AAO stand for in wastewater treatment?

AAO stands for Anaerobic, Anoxic, and Oxic. Each stage uses different microorganisms to remove pollutants from water.

Why is AAO treatment preferred for nutrient removal?

AAO treatment removes nitrogen and phosphorus efficiently. It uses biological processes, which reduce chemical use and save energy.

How does Mejec support decentralized sewage management?

Feature

Benefit

DeepDragon™ tech

Smart operation

Targeted systems

Industry-specific needs

Mejec designs intelligent solutions for healthcare, education, hospitality, and real estate.

Post time: Feb-04-2026 athuor:Nancy
Nancy Marketing Specialist
Nancy is a seasoned international marketing director at Mejec, dedicated to building global client networks and driving the market adoption of sustainable water solutions. Her expertise lies in connecting cutting-edge technologies with commercial success across diverse regions.

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